Catalog #: UB-200
Di-Ub/Ub2 WT Chains (K48-linked)
Quantity: 25 µg
Linkage specific di-Ub is a substrate for enzymes that cleave the isopeptide linkage between two ubiquitin molecules. It can also be used to investigate mechanism of binding and recognition by E1 or E2 enzymes, deubiquitinating enzymes, E3 ligases or other proteins that contain ubiquitin-associated domains or ubiquitin-interacting motifs (UIMs). This product is formed with wild-type human recombinant ubiquitin and linkage-specific enzymes.
Product Information
MW: 17 kDa
Purity: > 90% by SDS-PAGE
Buffer: 50mM PBS (PH:7.0)
Use & Storage
Use:
Typical concentrations will depend on specific assay conditions and method of detection.
Storage:
Solubilized solution at –20°C. Avoid multiple freeze/thaw cycles.
Literature
References:
Buchberger A., et a. (2002) Trends.Cell.Biol. 12:216-221
Cook W.J., et a. (1992) J.Biol.Chem. 267:16467-16471
Fischer R.D., et a. (2003) J.Biol.Chem. 278:28976-28984
Tenno T., et a. (2004) Genes to Cells. 9:865-875
Varadan R., et a. (2002) J.Mol.Biol. 324:637-647
Varadan R., et al. (2004) J.Biol.Chem. 279:7055-7063
